We used two mutants of DMPK: myc-K110ADMPK, a kinase deficient form mutated at the ATP-binding site [28], and myc-ΔMADMPK, which lacks C-terminal residues 550-629 but retains kinase activity [22].
We used TG2-C277S as a transamidation inactive form and TG2-R580A as a GTP-binding deficient form having greater transamidating activity.
Cells expressing a GTP binding deficient form of TG2 TG2-R580AA) withighgh basal transamidation activity and a more extended conformation showed significantly increased cell death in response to oxygen-glucose deprivation; however, targeting TG2-R580A thethe nucleus abrogated its detrimental role in oxygen-glucose deprivation.
Again, the specificity of BiFC was confirmed with a DNA-binding deficient form of Exd.
We tried to validate the specificity of these interactions by using a DNA-binding deficient form of AbdA.
The specificity of this interaction was validated by the absence of BiFC between NvHoxE and a DNA-binding deficient form of NvPbx, showing that the formation of the cnidarian Hox/Pbx complex is DNA-binding dependent, as previously noticed in bilaterians (Hudry et al., 2011, 2012).
This all-penetrating, yet latent and unnoticed presence of the background of communicative action can be described as a more intense, yet deficient, form of knowledge and ability.
In the Mohists' defense, one might argue that all goods at least produce some general feeling of satisfaction, if not delight, and that things that do not genuinely benefit us produce at best only a transient, unstable, or otherwise deficient form of satisfaction or delight.
It persists within the human gut in a Ziehl-Neelsen negative "cell wall deficient form" [ 1, 13] which could be the potential source of antigens in the host that may direct auto-immune responses.
For example, as recently as the 1960s, most scholarly linguists believed African American Vernacular English to be a deficient, broken form of standard American English reflective of African Americans' lack of cognitive abilities.
However, few laboratories can successfully culture the cell wall deficient form of MAP that exists in humans [14], [30], [31], a process that may take up to 18 months.
