Phylogenetic hypotheses derived from mitochondrial gene sequences revealed a deep break for the Cathorops mapale group around the PNNT in northern Colombia (Fig. 1).
This result is unsurprising, especially given the results of a previous study, showing a deep break of mtDNA haplotypes across the Sanaga [ 23].
In addition, even if there is a lack of geographical or taxonomic structuration, one might notice that several haplotypes representing different species are separated from the central network by a deep break of multiple mutation steps.
In the playground you could play with matches and throw marbles at each other, and if somebody called you a ninny you'd suck it up, take a deep breath and break one of their arms.
If the news is of such import and urgency that it can't wait for a "better time," just take a deep breath and break in to whatever is going on with something like, "I need to speak with you, Jane, and I'm afraid it can't wait".
New Horizons is about to get a deep-space break.
A deep phylogeographic break in the region north of Loreto such as the one observed in Ligia (i.e., blue stars vs. hexagons) is reported for a lizard [49].
Analyses of mitochondrial DNA sequences revealed a deep phylogenetic break between reciprocally monophyletic populations of Réunion and Mauritius [ 25], and an estimated coalescence time to a common ancestor within the last 430,000 years.
A subsequent phylogeographic study, focussing on the SW Pacific, used rDNA internal transcribed spacer (ITS) sequences that provided better resolution and uncovered a deep phylogeographic break on the Great Barrier Reef with distinct northern and southern clades.
Other lizards associated with humid areas, such as members of the genus Iberolacerta, show a deep phylogenetic break between the NW Spain form (I. monticola) and the Pyrenean taxa (I. bonnali, I. aranica and I. aurelioi), suggesting similar patterns of isolation as in Z. vivipara albeit at longer time scales [ 33, 34].
