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We observed that only a deep margin for excision was significantly related to the rate of recurrence.
Risk factor analysis indicated that a deep margin for excision was significantly associated with the rate of recurrence.
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Deep margins for cases where the carcinoma was wild type for TP53 were analysed for expression of Ly-6D by QRT-PCR, as previously described (Nieuwenhuis et al, 2003; Graveland et al, 2009), using the two-standard curve method for data analysis.
None of the risk factors, except for a deep surgical margin for excision, were significantly associated with the rates of recurrence, metastasis, and patient survival.
Hence, ensuring the presence of a sufficient deep margin as well as an adequate lateral margin is essential during wide excision of AS.
Other indications recommended by the Panel for post-mastectomy radiotherapy included positive deep margins and, for two-thirds of the Panel, tumours greater than 5 cm regardless of the nodal status.
The high rate of false-positive tests obtained with the Ly-6D QRT-PCR suggests that there are probably normal squamous epithelia in the deep margins forwarded for analysis, and this was confirmed by light microscopy.
Chest wall radiation is advised if DCIS extends to the deep margin after a simple mastectomy.
Only a deep excision margin was significantly related to the recurrence rate.
Cases with at least one positive tumour-adjacent mucosal or deep margin were considered to have a positive molecular test.
Involvement of the deep margin after BCS is considered as a positive margin.
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