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The library was constructed using two restriction enzymes and provides a deep coverage of 1RS.
The library comprises two sublibraries constructed using HindIII and EcoRI and provides a deep coverage of about 14-fold of the 1RS arm (442 Mbp).
To obtain a deep coverage of lowly expressed genes, particularly Olfr genes, three cDNA libraries with different insert sizes were constructed for each RNA sample.
The two most common approaches here are either designed to achieve a deep coverage of the proteome (shotgun MS [ 5]) or to collect as much quantitative information as possible for a defined set of proteins/peptides (targeted MS [ 6]).
This materialized later with the publication of a deep coverage of waterhemp's transcriptome that yielded a total of 44,469 unigenes, 49% of which displayed highly significant similarities to Arabidopsis proteins [ 32].
Illumina HiSeq Sequencing was selected to provide a deep coverage of identified genes as the large number of reads allows quantification of gene expression by mapping to reference genome, and is usually sufficient to detect most expressed gene even at low expression level [ 25], which is an ideal choice at a cost-performance perspective.
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Assemblies were performed for each isolate individually and also as a combined assembly including all sequence data for a deeper coverage of the common features of the cluster SMI-049 isolates.
To achieve a deeper coverage of the intrinsic biogas community and to elucidate its whole complexity, the same community DNA preparation that was used for sequencing on the Genome Sequencer FLX was now sequenced on the GS FLX Titanium platform.
However, to better investigate and understand regulatory networks of such exemplified diseases in the porcine model, a deeper coverage of the intestinal miRNAome is needed.
The advantage of label-free LC-MS-MS/MS based quantitative glycoproteomics is the ability to compare an unlimited number of samples with a deeper coverage of identification compared to strategies that incorporate stable isotope labeling [ 37, 38].
Indeed, refinements to our palmitoylated protein enrichment protocol that included increased incubation time with streptavidin agarose and repeated washing steps, along with updates to our mass spectrometry capabilities, have led to a deeper coverage of the DC2.4 palmitoylome.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com