The phrase "a decrease of the annealing temperature" is correct and usable in written English.
It can be used in scientific or technical contexts when discussing changes in temperature during processes like annealing in materials science or metallurgy.
Example: "The experiment showed a significant effect on the material properties with a decrease of the annealing temperature."
Alternatives: "a reduction in the annealing temperature" or "a lowering of the annealing temperature."
For PCR amplification of rbcL DNA sequences, the same cycling protocol as for the ribosomal DNA sequences was used except for a decrease of the annealing temperature (predenaturation for 3 min. at 95°C; 30 cycles: 1 min. at 95°C, 2 min. at 45 or 50°C, 3 min. at 68°C).
The sequences of these primers are listed in Additional File 7. PCR experiments were carried out in a PTC-200 (Bio-Rad, Waltham, MA, USA), and Touchdown PCR reactions were performed under the following conditions: 94°C for 3 min; 20 cycles at 94°C for 50 sec, 65°C for 1 min, and 72°C for 50 sec, with a decrease of the annealing temperature of 0.5°C per cycle.
Above this temperature, the annealing rate demonstrated a sharp decrease as the annealing temperature approached the Tm, whereas, below this temperature, a comparatively modest decrease in annealing was observed.
For the selective amplification a touch down PCR with a temperature decrease of 0.6°C of the annealing temperature each cycle was applied.
With an increase of the annealing temperature, the Ti peak intensity decreases.
The slope of the C-V curve increases with the annealing temperature increases, indicating that the interface trap density decreases as the annealing temperature increases.
Q f decreases as the annealing temperature increases.
By decreasing the annealing temperature, a blue shift of emission peak for perovskite CH3NH3PbBr3−xClx layer can be observed.
In comparison to the (101) peak, the intensity of (002) decreases more when the annealing temperature increases.
However, further increase of the annealing temperature results in the decreased intensity despite increased size of Ag nanoparticles.
Increase of the annealing temperature to 300 °C resulted in significant decrease of the intensity of Raman scattering peaks.
