Exact(24)
To confirm that the decrease in expression of genes in the second and third waves in response to cordycepin was due to a decrease in transcription and not to the degradation of non-polyadenylated transcripts caused by residual cordycepin, we investigated levels of unspliced Mix1 RNA, using intron-specific primers (Materials and Methods).
Previous studies indicated that a decrease in transcription is involved (Epstein et al., Molecular Endocrinology 3 193-202, 1989).
For all the mutant strains a decrease in transcription was detected on wheat straw for all the genes tested.
In contrast, in a different study siRNA-mediated PP5 knock-down lead to a decrease in transcription of GR target genes [32].
Therefore, humans who abused cocaine, cannabis and/or phencyclidine share a decrease in transcription of calmodulin-related genes and increased transcription related to lipid/cholesterol and Golgi/ER function.
However, co-cultured macrophages showed a decrease in transcription compared to macrophages cultured alone.
Similar(36)
To determine whether the decreased production of MMP-2 was due to a decrease in gene transcription, MMP-2 transcripts were assessed via RT-PCR.
We observe that the hypermethylation of CAHM in colorectal adenomas and CRC is accompanied by a decrease in its transcription; this downregulation contrasts with elevated transcription of lncRNAs such as CRNDE and HOTAIR in CRC.
There was also not a decrease in MUC2 transcription and in NF-κB transcriptional activity (Fig. 7b).
The increase in Tat transactivation, however, was primarily due to a decrease in basal transcription, and only a small increase in Tat-dependent transcription [16].
As previously reported, MDG147 cells exhibited an increase in ompC transcription, as indicated by increased CFP fluorescence, and a decrease in ompF transcription, shown by decreased YFP fluorescence.
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