The quantities of DNA retrieved appear to decrease in a time dependent manner (Fig. 2).
The number of cells arrested in the G2/M phase in the presence of both PMA and apicularen A decreases in a time-dependent manner and leads to an increase in the number of cells in the sub-G1 phase.
Meanwhile, the protein content of MyHC was found to decrease in a time-dependent manner in C2C12 myotubes.
The levels of Pdx-1 protein in INS-1 cells treated with GS also appeared to decrease in a time-dependent manner (Fig. 2B).
Circulating fetuin-A levels were decreased in a time-dependent manner, starting between 26 h, reaching a nadir around 24 48 h, and returning towards base-line approximately 72 h post onset of endotoxemia or sepsis.
Curosurf® exposure resulted in a time dependent decrease in cell viability in all cell models and a time dependent increase in proliferation in A549 and RPII cells, but a time dependent decrease in iMATII cells.
In Tet off cells, administration of CHX (50 μg/mL) caused a remarkable decrease of Smad3 in a time dependent manner (Fig. 1C and 1D).
Surprisingly, total protein synthesis decreased dramatically in a time dependent manner, and was reduced to less than 1% after 24 hours (Fig. 2A and 2B).
Moreover, a decrease in OSX mRNA expression was revealed in time-dependent experiments with BMSCs.
We monitored cellular proliferation after miR-145 upregulation in PC3 cells and found that cell viability significantly decreased in a time-dependent manner in miR-145-transfected cells compared with the negative control and mock samples.
