Your English writing platform
Discover LudwigExact(4)
Had I bought a daily medium latte at Stumptown for 72 days, I'd have spent $324.
In general, treatment of cells was similar in dose response experiments, except for a daily medium exchange.
The medium was replaced with normal growth medium and the cells were incubated in appropriate conditions for 72 hours with a daily medium refresh after which the cells were harvested for Western blot and RNA expression analysis.
The culture was maintained in complete medium (DMEM, 10 mM HEPES, 1 mM pyruvate, 2 mM glutamine; Gibco BRL), 100 U/ml penicillin (Jenapharm, Jena, Germany), 0.1 mg/ml streptomycin (Gruenenthal, Stolberg, Germany) and supplemented with 20% FCS (Gibco) for seven days in tissue culture plates in a 5% CO2 atmosphere at 37°C with a daily medium exchange.
Similar(56)
Cells were grown for 3 to 5 days at 37°C in a humidified 5% CO2 atmosphere, with daily medium changes including a final medium change two hours prior to running the assay.
A daily full medium change was performed with warm STEMdiffTM Neural Induction Medium.
After 48 hours, the cells were transferred into T75 flasks at a density of 2 × 10 cells per flask, and 5′-azacytidine (Sigma-Aldrich) was added at a final concentration of 10 μM for 96 hours with daily medium changes.
Once daily, medium was changed and excess mucus was removed by washing with a micropipette.
All conditions were incubated for 72 h with daily medium change.
The cells were grown for additional four weeks under daily medium change.
Without passage, E14 cells cultured in mES medium for a long period tend to spontaneously differentiate even in the presence of LIF and with daily medium change.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com