Exact(3)
PCR was carried out using a cycling sequence of 95°C for 5 min followed by 35 cycles of 95°C for 1 min, 45°C for 1 min and 72°C for 2 min, which was then followed by a final extension of 72°C for 10 min. New sets of primers were designed from the sequence analysis of these virus-specific cDNAs for determining the remaining sequence.
"Big Shots" on ABC-TV moved a cycling sequence from Malibu.
Other tracks use a cycling sequence of chords as a structure for small amounts of improvisation, or written parts played in a loose, improvised manner.
Similar(57)
This criterion was stringent as it excluded 5 of the 205 sequences in the training set as well as the cycling sequence binding protein A (CSBPA) of Lm; the Tb and Tc orthologues of CSBPA were recognized.
The approach is illustrated through several test cases, in which different combinations of objective and constraint functions, design variables and cycling sequence patterns are examined.
Cycling sequence was performed with Big Dye Terminator Version 3.1 kit (Applied Biosystems, Foster City, CA), following manufacturer's instructions.
This motif is recognized by the Cycling Sequence Binding Proteins CSBPA and CSBPB [13] and also CSBP II [14].
For clusters #3 and #6 (which had similar expression profiles), analysis with Trawler yielded motifs that were variants of the well characterized canonical Cycling Sequence (CS) with consensus [CAUAGAAG] [12].
The [CAUAGA] motif of late G1-upregulated clusters #3 and #6 (Fig. 6), homologous to the well-characterized canonical Cycling Sequence (CS) of kinetoplastids [12], is known to be recognized by the CSBP II complex that mediates regulation of a cohort of transcripts that mostly encode proteins involved in DNA metabolism.
Tb: Trypanosoma brucei; Lm: Leishmania major; Tc: Trypanosoma cruzi; Lb: Leishmania braziliensis; Li: Leishmania infantum; Tc: Trypanosoma congolense; CSBP: cycling sequence binding protein.
A suspected sample was subjected to direct sequencing using the cycling sequencing system (Applied Biosystems, Foster City, CA, USA) and run on Perkin-Elmer ABI-377 automatic sequencer.
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