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To probe miRNA differential expression in CTGF-treated OASFs, we used a customized array of 96 human miRNAs.
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Results were confirmed by a customized array using most of the PAM-selected genes.
Of 2054 selected genes on a customized array, 317 were differentially expressed (P < 0.05) in Xcc challenged kumquat plants compared to mock-inoculated ones.
A customized array representing 3359 water stress responsive genes was designed to document their expression in leaves of E. grandis cuttings subjected to − 0.225 MPa of PEG treatment.
Further, a customized array design can provide comprehensive genomic profiling with additional accuracy in both identifying and defining the extent of small aberrations at target loci.
Microarray analysis offers a customized array containing genes specific to particular cell populations or signaling pathways.
This is made possible by using a microfluidic chip integrated with customized arrays of permanent magnets capable of creating large magnetic field gradients, which determine the effective capturing of the tagged cells.
TALENs consist of two functional domains: a customized repeat array of TAL DNA-binding domains that recognize a specific DNA sequence and nuclease domain of FokI, a class IIS restriction enzyme (Miller et al., 2011).
Additionally to these known miRNAs, the customized array contained a set of 85 sRNAs of unknown function that were derived from an earlier high-throughput sequencing experiment of phloem sap [ 1] (sequences and accession numbers in additional file 5).
MIRACLE is designed with user approachability in mind, but also supports R data analysts by offering a convenient data export/import interface with R. While the data analysis part of MIRACLE is particularly laid out for handling RPPA data, the sample management functionality is suitable for any kind of customized array design.
All these data supported the reliability of our customized array platform and the subsequent analyses for identifying canine CNVs, therefore we applied this system to analyze cloned dog genomes.
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CEO of Professional Science Editing for Scientists @ prosciediting.com