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These autogrids were mounted into a dual-beam (FIB/SEM) microscope (Quanta 3D FEG, FEI) using a cryo-transfer system (PP3000T, Quorum) with a custom-built transfer shuttle.
Subjects were asked to position themselves next to a bench, which was at a height level to their own wheelchair seats, on a custom-built transfer station [ 33].
The design and testing of a circular glow discharge ion source on a custom built proton transfer mass spectrometer are described.
After each process, the sample was carried to a surface analysis chamber for X-ray photoelectron spectroscopy (XPS), using a custom designed in-vacuum transfer device.
Figure 3 Design of a custom MapReduce job to transfer clustering output to HBase.
In a custom that has been transferred from naval ships to space stations, Fyodor N. Yurchikhin, the station commander, rang a bell to signal the arrival of the new guests.
The intensified image was acquired using a custom, 16 bit, frame-transfer charge coupled device (CCD) camera and the images were saved onto a computer.
The diaphragm was bisected, and one half was transferred into a custom recording chamber in which the muscle was held in place with metal pins that passed through the surrounding tissue and were inserted into a Sylguard bed.
For live imaging of notochord cells within mGFP- or mcRFP-morphant embryos, the chorion was first removed with forceps at 90% epibody/bud stage and embryos transferred to a custom acrylic chamber filled with E3 solution.
Transverse hippocampal slices (350 µm) were transferred to a custom made interface type recording chamber and incubated for at least 2 hours at 32 degree under constant perfusion with carbogenated artificial cerebrospinal fluid (ACSF containing in mM: 110 NaCl, 5 KCl, 2.5 CaCl2·2H2O, 1.5 MgSO4·7H2O, 1.24 KH2PO4, 10 glucose, 27.4 NaHCO3, pH 7.3).
Output data generated by the instrument on-board software Sequence Detector Version 1.6.3 (PE Applied Biosystems, Foster City, CA) were transferred to a custom designed Microsoft Excel macro for analysis.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com