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This was followed by a coupling step where molecules with pyrene groups were allowed to react with amines.
This was achieved by increasing the hydrophobicity of the polymers in two distinct steps, namely: macromer formation and a coupling step.
In a coupling step, N-hydroxysuccinimide-activated Cy3 and Cy5 dyes (Amersham Bioscience) reacted specifically with the modified dUTPs in the cDNA, producing labelled probes.
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Oxidation of NADPH by oxidized glutathione was then recorded photometrically in a coupled step (λ=340 nm).
For a couple steps.
Yeah, and take a couple steps back.
"It seems like the economy takes a couple steps forward and then a couple steps backward".
And then, after a couple steps, he was not.
In Midtown, a couple stepped out of a taxi after a ride from La Guardia Airport.
"I had to take some time off; I took just a couple steps back," Williams said.
"He's always been a couple steps ahead of everybody," Brown said.
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