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Thus, the lack of a correct integration between these infrastructures and Kerberos limits the service access only to service provider's subscribers.
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Obtaining a product from such a PCR indicated correct integration of the knock-out cassette.
(A ) Southern blot verifying correct integration of the targeting construct.
(A ) Southern blot verifying correct integration of the YapKI targeting construct into the Gt ROSA)26Sor locus.
Additionally, a fragment indicative of the correct integration event could also be detected (Fig. 3A).
Rate of correct integration was comparable to a previous study using similar condiitons and was in the range of 40 80% depending on the gene locus considered [ 26].
The probe was hybridized to a human male metaphase to check for probe intensity and correct integration site.
The most convincing evidence for the correct integration into the DHFR-TS locus is a loss of the DHFR-TS activity in the transformed strain.
LEDGF is also a binding partner of HIV integrase and is required for correct integration of the viral genome into the host chromatin [ 30].
Nevertheless, the correct integration of such parameters and their exposure as a clinical guide are still unresolved issues.
Colonies displaying an erythromycin resistant and chloramphenicol sensitive phenotype were checked for correct integration by PCR, using primers annealing outside of the region of integration in the chromosome (control primers in Table 4) and primers annealing in pORI28 (pORI28_for and pORI28_rev).
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