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We're sold the idea of startups as a cool step towards wealth and fame but the bulk of self-employed are tradies and corner shopkeepers, as well as more upmarket barristers and medical specialists who traditionally work freelance – not hipster Silicon Valley types.
If you can take a cool step back, it's a good message.
The winning concept of Watershed's Playable City Award, whose self-stated goal is to imagine, "a city where people, hospitality and openness are key, enabling its residents and visitors to reconfigure and rewrite its services, places and stories," even if you're afraid of your own shadow, these friendly silhouettes are a cool step toward making the city of Bristol even more stimulating.
You can add your own flare to this to make it unique, like a cool step with the feet.
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follows a cooling step of the humid air providing potable water as a condennsate.
First the pipe is placed inside an oven to undergo the heating stage, which softens the material thus allowing the deformation phase to be carried out by means of pressurized air or mechanical devices; the end of the process is a cooling step which freezes the pipe in its final shape.
The long-term cooling trend that led to the polar climate of today began with a cooling step ∼41 Ma during the middle Eocene.
Conditions for the RT-qPCR were a pre-denaturing step of 95°C for 10 min, 45 cycles of; 95°C for 10 sec, 60°C for 30 sec, 72°C for 30 sec and finishing with a cooling step at 40°C for 1 min. The quantification cycle (Cq) for each signal was calculated based on the Second Derivative Maximum method.
The PCR protocol consisted of denaturation program (95°C for 10 seconds), 40 cycles of amplification and quantification program (95°C for 10 seconds, 60°C for 20 seconds, 72°C for 15 seconds with a single fluorescence measurement), melting curve program (60 95°C with a heating rate of 0.1°C per second and a continuous fluorescence measurement) and finally a cooling step to 40°C.
Cycling conditions were as follows: denaturation (95°C for 5 min), amplification and quantitation (95°C for 10 s, 60°C for 10 s and 72°C for 15 s) repeated 40 times, a melting curve program (65 95°C with a ramp rate of 2,2°C/s) and a cooling step to 4°C.
The last step was a cooling step at 40 °C for 10 sec.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com