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The DYM gene was used as a control to normalize the expression levels.
Another pair of primers amplifying an unrelated gene was used as a control to normalize the qRT-PCR results.
Each sample was run in duplicate and β-actin was used as a control to normalize for differences in the amount of total RNA in each sample.
GAPDH was used as a control to normalize the data.
RUN6B small nuclear RNA was quantified as a control to normalize differences in total RNA levels.
The U18 or GAPDH gene was used as a control to normalize any differences in the total RNA levels in each sample.
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Aldolase A was used as an internal control to normalize for sample to sample variations in total RNA amounts and for reaction efficiency.
The empty pcDNA3 was used as a vector control to normalize the total DNA for transfection.
Densitometry analysis of a DpnI resistant band on the agarose gel prior to transfer was used as a loading control to normalize values obtained from the Southern blot.
GAPDH (1 1000) and alpha tubulin (1 1000, Santa Cruz Biotechnology) was run as a loading control to normalize samples.
Expression of β-actin was used as a qualitative control to normalize the protein levels for the Western blot procedure.
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