Glucokinase (hexokinase IV) has a major role in the control of blood glucose homeostasis because it is the predominant hexokinase expressed in the liver, has a very high control strength on hepatic glucose disposal, and is the glucose sensor for insulin secretion in pancreatic β-cells.
Then data were filtered using the control strength, a control value calculated using the Cross-Gene Error Model based on replicates.
Then, data were filtered using the control strength, a control value calculated using the Cross-Gene Error Model on replicates [ 14] and based on average base/proportional value.
NCA is a decomposition of the data matrix E into the control strength matrix A and the TFA matrix P through minimizing the residual Γ.
The criteria can be summarized as: (i) The control strength matrix A must have full-column rank; (ii) When a node in the regulatory layer is removed along with all of the output nodes connected to it, the resulting network must be characterized by a connectivity matrix that still has full-column rank.
The inferred control strength matrix A is listed in Table 2 and Additional File 1: Table S4.
Following similar procedures as discussed in Cases I and II, these genes are then applied to construct the matrix E and the connective structure of the control strength matrix A through the gene-TF database.
These data may also support a developmental switch in the control strength of mitochondrial transfer RNAs and, in particular, mt-tRNAGlu in mitochondrial translation, suggesting that 16 30% steady-state levels of mt-tRNAGlu may have a profound effect on translation in muscle of neonates but may become less critical at later stages of development.
Moreover, an adaptive algorithm for the control strength is also proposed to realize the quasi-synchronization.
From the best available evidence it appears that at MMR all five of the major steps in energy delivery (namely, ventilation, pulmonary diffusion, cardiac output, tissue capillary – mitochondrial O2 transfer, and aerobic cell metabolism per se) approach an upper functional ceiling, with control strength being distributed amongst the various O2 flux steps.
