Because the active control bottle (Xalatan) was visibly different than the investigational bottles, a designee at each study site, other than the investigator, was responsible for the dispensing study treatment at Visit 3, instructing patients on proper instillation of study medication, and retrieval of materials at the end of the study.
On day 1, 500 mL algae with a concentration of 450 \(\mu g/L\) were mixed with water and particles to a total volume of 1 L in four different test bottles (except for the control bottle, which received only water).
On day 1, 100 ml algae or water were added to each bottle together with particles with different concentrations except for the control bottle, which received only water (Table 1).
Each test consists of four treated bottles and one control bottle coated with acetone or ethanol only.
Two control bottles, one with 1% control (0.5 ml of the 1 100 diluted test organisms suspension) and one original control bottle without drug were used for interpretation of the test results.
The participants' behaviour towards the odorized and control bottles was measured in terms of mouthing, a behaviour considered to reflect attraction.
For larval feeding, 0.5ml of 3.2mg/ml RU486 stock solution (or the indicated diluted concentration) was added to the surface of each bottle to produce a final concentration of ~160ug/ml (or indicated diluted concentration); 0.5ml ethanol was added to control bottles.
In this investigation, the effect of amount of transverse reinforcement on strength of steeply inclined normal-strength and high-strength concrete bottle-shaped struts measured in terms of efficiency factors has been experimentally investigated for three transverse reinforcement ratios - 0.0031, 0.0046 and 0.0069, with the control bottle-shaped strut being transversely unreinforced.
Open symbols indicate average of replicate uninoculated negative control bottles.
Un-inoculated control bottles were kept to account for the abiotic loss of BDE 209.
Four to six replicates were performed for each concentration and for the control bottles (impregnated with acetone).
