Getting "straight in" is not a continuous requirement, but merely an opening gesture of intent, which is likely to be unfulfilled.
Furthermore, since re-introduction of miR-142-3p miR-142-3p miR-142-3prentintod MKs restored functional identity, we conclude that there might be a continuous requirement for miR-142-3p activity to maintain MK maturity.
Chronic infection with the intracellular protozoan parasite Toxoplasma gondii leads to tissue remodelling in the brain and a continuous requirement for peripheral leucocyte migration within the CNS (central nervous system).
Here we demonstrate a continuous requirement for β-catenin in the establishment and maintenance of acinar cell mass, extending from organogenesis in the embryo through expansion and homeostasis in the juvenile and adult organ, as well as regeneration following injury.
Together, this work identifies a continuous requirement for β-catenin in acinar development, extending from specification in the embryo to self-renewal in the juvenile and regeneration in the adult.
In any event, the overall agreement between these results and our findings in neonatal and uninjured adult mice indicate a continuous requirement for β-catenin in acinar cell proliferation, during both physiological and regenerative growth.
Patients with type 1 diabetes (n = 334) defined as acute presentation with diabetic ketoacidosis, heavy ketonuria (>3+), or a continuous requirement of insulin within 1 year of diagnosis were excluded from this analysis.
Importantly, the new sets of experiments allowed to make a new and important conclusion in the Results section: "Furthermore, since re-introduction of miR-142-3p miR-142-3p miR-142-3prentintod MKs restored functional identity, we conclude that there might be a continuous requirement for miR-142-3p activity to maintain MK maturity".
A continuous requirement for RNF12 for the activation of Xist and for maintenance of XCI may also explain why Rnf12+/− female embryos which maternally inherit the mutated allele display severe growth defects: maternal inheritance of the mutated allele would result in an Rnf12 null embryo because the wild type paternal X becomes inactivated during imprinted XCI.
Dealing with daily problems might become more complicated for the parents due to continuous requirements of the diabetes therapy and lack of social support.
