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Subsequently we used the OverUnder algorithm [16] to correct the hybridization log ratios for polyploidy and to attribute a continuous copy number value to each SNP.
As such, tissue mosaicism can occur over a continuous spectrum (1-99%), and can result in extensive overlap between integer classes resulting in data that seemingly indicates a continuous copy number distribution.
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The association between copy number levels and expression estimates was quantified by fitting a linear model between continuous copy number estimates (segment means) and log2-transformed normalized expression estimates and testing the slopes for significance.
Given that the heatmap is constructed from aCGH copy number states rather than continuous copy number ratios, these differences could be due to artificial changes in ratios near the cutoff for defining gains or losses.
Similar to their approach, our GMM implementation produces (continuous) copy number dosage values that account for uncertainty in the prediction (e.g. due to sample contamination or tumor cell heterogeneity).
Our PCA-merging method allows 1) reconciliation of 'disrupted' CNVs, 2) to consider the predicted copy number value without loss of information due to subsequent discretization (i.e. use of continuous copy number prediction) and 3) to ignore (outlier) variation likely induced by noise in the measurement.
With such a tradition, the survival of Chinese texts was assured by continuous copying and was not dependent on the capacity of a lone example to withstand the wear of the centuries.
Three classes of AU-rich sequences have been described including those with single or noncontinuous canonical UUAUUUAWW motifs (Class I), those containing overlapping or continuous copies of the motif (Class II), and a 3rd class of U-rich sequences lacking that the AU-motif (Class III) [15] [17].
The survival of Chinese texts was assured by continuous copying.
We found that overall, real-time QPCR produced a continuous spread of copy number values rather than discrete clusters.
Association of SLC2A3 copy number with HD AO was tested by generating a generalized linear model using SPSS 20 (IBM), with CAG as a continuous predictor, SLC2A3 copy number as an ordinal predictor and log2AO as the continuous response variable, using Wald Type III analysis of variance (ANOVA) statistics.
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