Towards this goal, we also constructed transcriptome viewer in GBrowse format to facilitate visualization of all uniquely mapped mRNA-Seq reads in the rice genome under Pi stress (Fig. 6; http://rnaseq-pi.dna.affrc.go.jp/cgi-bin/gb2/gbrowse/RNAseq_p/).
We constructed transcriptome libraries from two tissues of A. segetum: pheromone gland (As_PG) and abdomen (As_AB).
We determined gene expression profiles by BeadArray™ [ 17] and constructed transcriptome maps for both ES and EB cells.
The genome data of WSSV (NC_003225.1) and the constructed transcriptome data of F. chinensis were used to screen potentially interacting proteins by searching in protein interaction databases, including STRING, Reactome, and DIP.
The algorithm performs several functions such as aligning the sequence reads to a genome, constructing transcriptome maps, calculating the abundance of transcripts, differential gene expression, and predicting transcription unit structure.
In the present study we de novo constructed transcriptomes of three Chinese wild grapes, which showed resistances to various fungal pathogens.
To detect AS events during wood formation, we first constructed transcriptomes of developing xylem in Populus (P. × euramericana) and Eucalyptus (E. grandis).
It could serve as a model tool to construct transcriptome databases for those organisms without complete transcriptome information.
Analyses of the six transcriptomes obtained result in 12,219 genes, recovered from a constructed reference transcriptome.
In this study, leveraging the newly available genomes and using deep RNA sequencing, we construct transcriptome assemblies and gene models — predicted genes each coding a family of transcript isoforms — for CM and AG, obviating the need for computationally expensive and less reliable de novo transcriptome assembly [ 20, 21].
RNA was fragmented with RNase III, preparing for constructing transcriptome libraries of each iPSC cell line.
