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Following silencing with a construct design that targeted the full length DSPP, only very low levels of the 97KD band was seen in most of the stable lines, indicating the silencing of DPP and full length DSPP.
(a) Construct design and protein sequence used to express and purify IL2-FKBP.
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The main contribution to feature generation studies is the notion of analytical features (AFs), a construct designed to support the representation of knowledge about audio signal processing.
Stronger knock-down of MYB75 was obtained from co-suppression of the endogenous MYB75 by a construct designed to overexpress MYB75 cDNA.
Here, we demonstrate the therapeutic potential of a TRAIL-based gene therapy approach utilizing a construct designed to deliver a secretable form of trimerized TRAIL using a vector system for local delivery.
Since we have used a construct designed for single cross-over integration, reversion of the disrupted locus to wild type can occur at low frequency in the parasite population as has been reported for P. berghei TRAP mutants [37].
This idea fits with the failure to rescue the endoA nulls that was also observed for the endoAP143A, ΔLEN transgene, a construct designed to decrease the curvature of the BAR domain without perturbing the central appendage.
We engineered a construct designed to express Twist1 tethered by a flexible linker to E47: we refer to this heterodimeric fusion protein as TwistE.
A final control consisted of a construct designed for the overexpression of the Arabidopsis NPR1 protein translationally fused to EGFP (35S: AtNPR1: EGFP).
Because of this, a construct designed to retarget the Ll.LtrB intron to insert within IS605 needed to be created in three steps.
Plasmid pLEW13, a construct designed to target the T. brucei β-tubulin locus, contains both T7 RNA polymerase and tetR genes with neo as a selectable marker [ 7] (Fig. 1A).
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