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To increase expression levels of the target protein, we designed a construct containing two tandem repeats of the target gene.
HEK293T cells were transfected with a construct containing the luciferase gene driven by the TEF promoter.
The first two groups comprised DNA plasmids that were derived from either a construct containing a 657 bp Dβ1-Jβ1.1 insert (group I; Figure S6) or a construct containing a 580 bp Dβ1 overlapping insert (group II; Figure S7).
Thus, pMEF2FLuc, a construct containing luciferase downstream to a promoter with MEF2 binding sites, was introduced into HeLa cells.
In addition, a construct containing the full length HFE C282Y mutant was made to be used as a dysfunctional control.
A construct containing the NTD of Mtbmfd was generated by cloning the F1 fragment into pET14b using NdeI-KpnI sites.
We used a construct containing the entire EN protein as bait to screen an embryonic cDNA library.
Nipponbare) was also transformed with a construct containing the CaMV35S promoter fused to the AtHKT1 1 gene to produce rice plants expressing AtHKT1 1 constitutively.
We chose to make a construct containing a GFP gene with 4 introns and 5 exons (the same construct as described previously [21] but with introns).
We then designed a construct containing the coding sequence, a loop of nine bases, the antisense sequence of the coding region and a termination codon (Figure 1A).
A construct containing the mouse aP2 promoter/enhancer region was used to generate a mouse model with adipose tissue specific expression of hSAA (Figure 1).
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