SV40 ori competition for T-Ag in the 293T cells is not the cause, as care was taken to maintain a constant ratio of origins in the various transfection cocktails.
Consider a constant odor source with an emission rate c(t) (ng/min) starting at t = 0 at the origin.
We propose a solution that treats the local vertical datum as a constant surface at the origin but varying elsewhere due to distortions in the local vertical datum.
If, instead, the primary signal is generated at a constant rate at the origin, the secondary signal moves more slowly than the primary signal at early times and moves more quickly at later times, a temporal order that is opposite that of the original result (Figure 9).
The open kitchen connects guests with the unrelenting chefs, who seem like they are cooking for you and you alone, while the scenery is a constant reminder of the simple origins behind the chefs' intricate preparations.
His years there were probably unhappy because, although he was proud of his middle-class origin, he was a constant butt of ridicule because of his ungainly figure, morose manner, and biting tongue; the bitterness of his book reflects the inferiority of his social position.
A constant rate of movement from the origin of each order to its final position was assumed.
It can therefore be assumed that U g on the plate is a constant and is evaluated on the origin x = 0, y = 0.
To test if the selective history of the populations was changed by laboratory rearing conditions (e. g. inbreeding or genetic drift), we compared the results of these recently collected populations to those of a long-adapted laboratory strain from a temperate origin, kept at a constant temperature of 18°C for 12 years.
Although all strict molecular clock studies (i.e. based on a constant molecular rate) have placed the origin of bilaterians before the first undisputed fossil evidence in the Cambrian, these studies have been criticized for many reasons, particularly because evidence suggests that rates of molecular evolution vary widely between different animal lineages (e.g.,[ 21]).
The B1 empty plasmid controls were included to maintain a constant quantity of DNA and SV40 origins to control for non-specific effects on transfection efficiency and origin replication.
