We previously have shown that, in addition to a quantitative change in TNC in breast cancer, there is a consistent change in the pattern of TNC isoform expression, with induction of two additional isoforms – TNC-16 and TNC-14/16 TNC-14/16detected in norarelyesting breast [ 28].
This also suggests a consistent change in the transcriptome of the biological replicates (within each set), which therefore seems to be related to the effect of the introduced gene(s).
In each case, the majority of genes that showed a consistent change had a higher level of expression in the negatively selected population.
It is promising that Pechal et al. (2013) recently discovered a similar consistent change of skin bacterial communities on three decomposing swine in an outdoor setting.
A failure to demonstrate a consistent change may reflect a number of issues.
Selection of differentially expressed protein spots was based on fold change abundance >2.0, with a consistent change in the replicate gels of the two biological replicates.
Although a more detailed functional analysis would improve the paper, one relatively simple suggestion is to determine more carefully whether or not there is any gross anatomical change in brain size or cortical thickness, or a consistent change in the soma size of affected hippocampal neurons.
However our findings of a consistent change in MAO-A, but not MAO-B, in conjunction with the literature provide some support for our premise that the critical role of MAO-A in cancer occurs via the serotonergic system rather than the dopaminergic or catecholamine system.
A recent meta-analysis [ 16] showed that the results are very consistent for N0 ER-positive disease in countries with different therapeutic traditions with a consistent change in treatment recommendations in ∼35% of cases.
Even though this study was not designed to investigate changes of patient's internal frame of reference with time, our results do not show evidence of any consistent changes of such an internal reference.
