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Since a large number of statistical test were involved a conservative level of significance was preferred.
Predictors were initially considered for regression modelling if they showed an individual association with an outcome at a conservative level of significance (p ≤.25) (Hosmer & Lemeshow, 2000).
By genotyping our Canadian RA case/control cohort for 101 SNPs across the locus, we have identified six variants for which association with RA reaches a conservative level of genome-wide significance (p<5.7×10−8).
> -wrap-foot> > -wrap-foot> > -wrap-foot> Varinnts in or near the FSHB gene were the only ones that reached a conservative level of genome-wide significance in the GWAS for menstrual cycle length (Fig. 2).
In order to set a conservative level of relatedness, we include 3 chromosomal fragments of 15 kbp with low δ* values compared to the complete chromosome; i.e. 3 different fragments with δ* values lower than that of 5%, 10%and25%5% of all chromosomal fragments of 15 kbp, respectively.
Six species had distinctive intraspecific clusters with probabilities of chance reciprocal monophyly below a conservative level of α = 1%: Kittlitz's Murrelet (Brachyramphus brevirostris), Gentoo Penguin (Pygoscelis papua), Gull-billed Tern (Gelochelidon nilotica), Eastern Meadowlark (Sturnella magna), Common Redshank (Tringa totanus), and Little Penguin (Eudyptula minor, Table 3, Figure 6).
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Methods chosen to control for multiple comparisons allow a moderately conservative level of control, and reflect the exploratory nature of the study.
The perceived associations were very strong and taking a highly conservative level of significance did not affect the results.
For those data sets for which the transformation did not allow to obtain homogeneous variances, a more conservative level of significance was considered [38].
Note that other authors suggest a more conservative level of differences of 0.30 that was not employed here [ 35].
Since we used a rather conservative level of significance as compared to previous VBM studies [ 1, 5, 19] this might have led to a false negative finding.
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