Implicit in this simplification is a crucial assumption that has never been critically evaluated in a broad context: Detailed mechanisms of protein folding are not biased by nonnative contacts, typically argued to be a consequence of sequence design and/or topology.
By analogy with the quinolones, this may reflect a consequence of sequence-selective DNA cleavage by topoisomerase II in the context of the drug/DNA/enzyme complex [40], [45].
Gene silencing as a consequence of sequence duplications is particularly prevalent among plant species.
Nevertheless, many sequence variants were detected reasonably well in the current study, suggesting that degradation of mRNA occurred rarely, if at all, as a consequence of sequence alterations.
Alternatively, differences between DNase I, Benzonase and Cyanase can be a consequence of sequence specificity for DNA recognition and cleavage by each of the endonucleases.
While this approach reduces the complexity of the pool of sequences hybridized to the microarray, differences in signal intensity that arise as a consequence of sequence variation among genotypes can be confounded with differences in transcript abundance.
These mutations may have arisen due to strain passaging or a consequence of sequencing errors from the original sequencing but the observation of these mutations clustering at loci suggests due to passaging.
However, some contigs in groups with FPKM>0 in E. grandis did not map to the genome sequence, which may be a consequence of sequence gaps in the E. grandis genome assembly.
This is a higher number of OTUs than previously reported to be associated with human gut [ 26, 29- 32], but we suspected this to be a consequence of sequencing error combined with the deep level of coverage [ 33].
Five of the 21 markers screened during primer development were thus rejected, and their failure to cross-amplify is likely a consequence of sequence divergence between these two lineages in the primer binding sites.
