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Exact(7)
After normalization of data in MS mode, the distribution of TTP correlated well with the distribution pattern obtained by MS/MS imaging on a consecutive section.
A consecutive section from each sample processed without the primary antibody was used as a negative control.
For each section which had areas of necrosis, a consecutive section was incubated with the antiserum against non-related bacteria as negative control.
As negative control, a consecutive section of the positive control was incubated with a polyclonal antiserum directed against non-related bacteria (Brachyspira hyodysenteriae) instead of the polyclonal rabbit anti-MAP serum.
As negative reference for the confocal analysis we used a consecutive section that was stained omitting the primary antibody anti-rat macrophages/monocytes and replacing it with equivalent concentrations of the unrelated IgG of the same subclass.
For a negative control, a consecutive section from the same specimen was not stained with the primary 53BP1 antibody and instead was replaced by non-immune rabbit IgG at the same concentration of the primary antibody.
Similar(53)
Every second of all consecutive section was analyzed.
(A) Two consecutive sections of a breast tumour TMA were stained with the same concentration of specific FMRP affinity purified antibodies (Ferrari et al, 2007) or the corresponding affinity-purified pre-immune IgGs.
Guided by an H&E stained consecutive section, the tumor area of each section was delineated.
For a control consecutive sections were stained with murine pre-immune serum to test for the specificity of the detection system (examples are shown in Figure S2).
From each paraffin-embedded sample, a 2-μm section was obtained for FISH analysis and a consecutive tissue section was H&E-stained in order to identify the tumoral and normal regions to be analyzed.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com