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It is unfortunately not possible to use in situ or Northern blot data to confirm the detailed cellular expression patterns of these transcripts, since there is no portion of any of them which is not also part of a different Y-linked gene or retrovirus with a confounding expression pattern.
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A major prerequisite for the current study was the ability to specifically target the salivary glands of larval flies without any confounding expression.
Despite this similarity, nucleotide mismatches between the species can confound expression analysis on commonly used microarray platforms.
A confounding fact is that the expression patterns of CEH-14 and RPGs are not related.
The distance of each CpG markers to the transcriptional start site (TSS) was used a covariate to fit into a linear model, but it was not identified as a confounding factor to influence gene expression.
The identification of differentially expressed genes, either from tissue to tissue comparisons or from smoker vs nonsmoker, highlights the effect of sequencing batch on gene expression, as a confounding factor.
Donor age could be a confounding variable in evaluating KIM-1 expression.
Because pieces of these tissues might contaminate the membrane during the dissection and confound a gene expression analysis, we evaluated them histologically.
Further confounding, the expression profile of these markers not only varies within areas of the same lesion but also during the course of disease in the same patient [ 8].
This variability needs to be recognized as an important confounding factor in expression analysis studies.
All Ethiopians were examined by 3 Ethiopian physicians, who accompanied us from Addis Ababa, to exclude disorders that might confound gene expression.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com