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We have also included the Fcγ locus, because it contains multiple associated variants, including a confirmed copy number variation (CNV) in SLE.
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We tested our method on three well-studied tumor-related aCGH datasets with experimentally confirmed copy numbers.
FISH with BACs RP11-185K20 (7.80 Mb; positions are given as BAC midpoints according to NCBI Build 36) and RP11-43B8 (7.76 Mb) from this region on normal lymphoblastoid interphase nuclei confirmed copy number differences were present between the homologous chromosomes (Additional file 2).
Bryant later confirmed copies of the identity documents have been sent to the Syrian consulate: "Hope these will be considered favourably by the C-G," he wrote.
10.7554/eLife.02137.011 Figure 4. Measurement of nuclear CENP-A confirms centromeric copy number.
qRT-PCR was also used to confirm copy number.
In three cases with marker chromosomes it confirmed the presence of additional genomic material and determined its size (Cases 5 and 29), but failed to confirm copy number gain in one case.
Therefore, further work to identify and characterize CNVs in human populations and confirm copy number variability is essential in order to better understand the significance of CNVs and to determine their role in common disorders.
We selected Sucrose Phosphate Synthase (SPS) because it is a confirmed single-copy gene for which good sequence information is available [ 17].
The investigation will focus on whether activation keys used to confirm a copy of a game is not pirated are (or have been) used for the purposes of geo-blocking — thereby limiting consumers' access to a purchased game, based on where in the region they are located.
I phoned him up to ask and was put through to a spokesperson, who confirmed that a copy of the letter I have in my possession was sent to Tower Hamlets council from Mike Weatherley's office.
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