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A confirmatory experiment was conducted to evaluate the accuracy of the optimized procedure.
A confirmatory experiment was performed to evaluate the accuracy of the optimization procedure and maximum fluoride removal of 88.78% was achieved under the optimized conditions.
Promising results were obtained from the optimization, and the results were validated using a confirmatory experiment demonstrating that the process could be effectively improved and that the optimization technique is robust.
A confirmatory experiment is conducted at optimal process parameters as documented in Table 11.
Expression levels were then validated in a confirmatory experiment by real-time RT-PCR using the same cDNA hybridized on the array.
In a confirmatory experiment, the macroarray was hybridized with labelled cDNAs from the same samples used for the construction of the subtracted library.
Similar(54)
Unfortunately, they did not perform any confirmatory experiment (real-time PCR or immunohistochemistry) in order to verify the reality of their microarray data [ 30].
A maximum carotenoids yield of 17.245 mg/L was demonstrated by confirmatory experiment to the optimum medium in 5-day fermentation.
The confirmatory experiment conducted with the optimal conditions provided an extraction yield of 73.4 ± 1.2% for D24 and 62.3 ± 1.8% for H13, corresponding to the maximum and showing that these conditions are valid regardless the composition of the used barley.
Table 11 Confirmatory experiment optimal process parameters' values Process parameter Optimal value A 140 °C B 176 °C C 166 °C D 8 RPM E 129 °C.
The confirmatory experiment described in the text used sdz-18 (RNAi) as a negative control and RNAi as in Oldenbroek et al. (2013); essentially identical results were obtained using the RNAi protocols described above and an empty vector negative control.
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