The other three temperatures were tested in a random order to control for temporal effects, with the bees allowed a minimum of 24 h habituation to a temperature condition prior to a test.
The fourth variable in our logistic regression model found predictive of fatal head injuries not identified in in other studies, was the lack of a musculoskeletal condition prior to a fall.
The effect of low HDL as an existing condition prior to 2,4-D exposure could not be tested in this cross-sectional study.
High body fat content was observed at the end of the rainy season, which supports the notion that individuals need to attain a sufficient physical condition prior to the long dry season.
It is hypothesized that these lemurs, like other Malagasy prosimians living in dry forest ecosystems, have to reach a sufficient physical condition prior to the dry season [3], [5], [31], [36] [38].
These results clearly highlight the importance of validating reference genes as the most invariant internal controls for a particular experimental condition prior to investigating the relative expression of target genes by RT-qPCR.
Our findings stress the importance of performing fundamental research, by validating the most invariant internal QPCR control(s) of a particular experimental condition, prior to investigating the expression of target genes examined by QPCR only, or as QPCR validation of microarray data.
Our applied study protocol most likely created an undesirable patient condition prior to major abdominal surgery.
Since movement intensity was below 50% of the maximum oxygen consumption in the current series of experiments, we doubt that such a low-intensity motor condition prior to encoding would affect encoding when a moderate exercise bout has no effect.
