Elute with three 25-ml portions of the ether, collect all of the eluate, transfer it to a concentrator, and concentrate to a volume of 4 ml).
The essential components of the system are a concentrator of high concentration ratio, a PV/T module and a tracking device.
A mixture of 200 300 pmol of the two labelled samples was concentrated in a Concentrator Plus (Eppendorf™, Hamburg, Germany).
A mixture of 350 400 pmol of the two labeled samples was concentrated in a Concentrator Plus (Eppendorf™, Hamburg, Germany).
Pro-prophenin 2 was dialyzed using 14 kDa cutoff dialysis tubing (Spectra/Por, Cat. 132678) and concentrated using a Concentrator plus (Eppendorf, Cat. 5305 000.304).
The eluted protein was buffer-exchanged into buffer C (0.1% DDM, 20 mM Tris, pH 7.5) and concentrated in a concentrator with a 100 kDa cut-off membrane (Millipore).
The SB hemicellulosic hydrolysate was vacuum concentrated at 70°C in a concentrator and further detoxified by sequential calcium oxide-activated charcoal pretreatment according to Alves et al. [ 52].
After the synthesis reaction, the reaction mixture was concentrated to 300 μl with a concentrator (Amicon Ultra-15, 30,000 MWCO, Millipore-Japan, Tokyo, Japan).
Peak fractions from the two runs were pooled (∼12 mL total at 0.55 mg/mL) and concentrated to 20 80 mg/mL using a concentrator (Amicon Ultracel-50 membrane, Cat. UFC905008, Millipore).
In 1989 a concentrator solar cell in which sunlight was concentrated onto the cell surface by means of lenses achieved an efficiency of 37percentt owing to the increased intensity of the collected energy.
The operation also includes a concentrator facility for producing lead, zinc and bulk concentrates.
