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The decoction extract was dissolved in a measured volume of water with a concentration equal to 10 mg of crude botanicals per milliliter.
The average Nusselt number increases for increasing particle concentrations and Reynolds numbers; moreover, the highest values are observed for H/W = 10, and a maximum increase of 18% is detected at a concentration equal to 6%.
The competitor (biotinylated AnnV: Annexin V-Biotin Reagent from CLINISCIENCES) was then added at a concentration equal to its Kd for PS (5 × 10−10 M) and incubated for 1 h 30.
The presence of Cu2+, Ca2+or Fe2+ at a concentration equal to their IC50 left the affinity for Mg-ATP unchanged or even slightly increased, as shown by the kinetics towards this substrate (Fig. 6B, Tables 3 and 4).
A control of 0.11 mM FeCl3 (final concentration) at pH 3.5 was also tested for antibacterial activity against E. coli ATCC 25922 to determine the toxicity of iron at a concentration equal to the level of iron in BY07 leachate.
Controls received dimethyl sulfoxide (DMSO) vehicle at a concentration equal to that in drug-treated cells.
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The supernatant was removed and the bacterial pellet was re-suspended in a saline solution (0.85% NaCl) and then diluted to a standard concentration equal to an optical density (OD) of 1.0 at 545 nm.
It should be taken into account that all the experiments were carried out by using 0.5 l of a PNP solution with a initial concentration equal to 80 mg l-1.
In order to apply Chou and Talalay method (Chou and Talalay, 1981, 1984; Chou, 1991), we first fitted dose effect curves for single inhibitors with Hill functions of the form E= C n / k n + C n ); here E is the percent effect deriving from a drug concentration equal to C of a single drug and k and n are coefficients to be fitted.
For this depth range, overall RMSE values range from 0.89 m to 2.62 m when using a chlorophyll concentration equal to 0.2 mg m−3 and a generic seafloor spectrum generated from a spectral library of common benthic constituents.
Specifically, the practice of "shock chlorination" calls for addition of free chlorine at a mass concentration equal to 10 times the combined chlorine signal from a wet-chemical method.
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