Exact(5)
After filtering a concentrated cell suspension, the filter was gently rinsed with buffer, carefully cut (1 cm ×1 cm), attached to a steel sample puck (Veeco Metrology Group) and the mounted sample was transferred into the AFM liquid cell while avoiding dewetting.
A concentrated cell solution was prepared from 400 ml of FeOB M. ferrooxydans PV-1 cell culture.
The mycelium was collected by centrifugation and resuspended in fresh TSB/YEME to yield a concentrated cell paste.
After rinsing 3 times with PBS, a concentrated cell solution comprised 2 × 10 preconditioned 3T3-L1s (CorTumoror Tumor) was seeded on the mica substrates.
Synechococcus sp. PCC 7002 cultures were harvested at an OD650 nm ranging from 0.8-1.0 0.8-1.0SpecThermoSpectronic3 spectrophotometer) via centrifugation and resuspended in 1/5 the original media volume, yielding a concentrated cell suspension.
Similar(54)
The formation of Cerenkov UV radiation by gamma irradiation can be blocked, e.g., by using a very concentrated cell suspension (self shielding), or by suspending the cells in a solution containing a UV radiation absorber (e.g., DNA).
C. reinhardtii cells grown to about 10 cells mL−1 were harvested by centrifugation and resuspended in fresh TAP medium to obtain a 100-fold concentrated cell suspension.
Figure 6 depicts the improved methodology, requiring a fivefold more concentrated cell inoculum in buffer added in one-tenth, rather than one-half, of the 100 µL total volume of the 2-hour incubation step.
High-pressure homogenization of a concentrated bacterial cell suspension in a French-press is an option for cell disruption.
Take 0.1 ml of the concentrated cell suspension and dilute it to an approximate concentration of 1-2 × 10 cells/mL.
Immediately prior to the labeling reaction, MgCl2, MnCl2, and CaCl2 were added to the concentrated cell lysate until their final concentrations reached 50, 5, and 5 mM, respectively.
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