The images of stained tissue were captured by the computerized image system composed of an Olympus CCD camera connected to a Nikon eclipse Ti-s microscope.
A computerized imaging system was used for all histological measurements (Qwin; Leica Imaging Systems Ltd., Wetzlar, Germany).
The thickness of the synovial tissue was measured with a computerized imaging system again in three sections per knee joint and averaged per joint as previously described [ 27] (Qwin; Leica Imaging Systems Ltd., Cambridge, UK).
The blots were then exposed to X-ray film and band positions and band densities were calculated using a computerized image analysis system (UVP Biochem Imaging systems, Upland, CA).
In addition, to estimate any treatment-induced changes in liver fibrosis, the fibrosis area was quantified as a percentage of the total area that was positive for MTC stain in the digital photomicrographs using a computerized image analysis system (Analysis 3.0, Soft Imaging System, Münster, Germany).
Labeled sections were evaluated and photographed using an Olympus BX51 fluorescence microscope fitted with specific filter systems and a computerized image analysis system (SPOT camera, Image Pro Plus software).
OD was obtained using an AxioSkop 2 microscope (Zeiss) and a computerized image analysis system (AxioCam, Zeiss), equipped with dedicated software (AxioVision Rel 4.2, Zeiss, Germany).
To overcome the limitations of manual assessment, the BSI methodology was automated using a computerized image analysis system that employed an artificial neural network [5].
Nonatretic antral follicles were isolated, measured with a computerized image analysis system and grouped according to their diameter: Group 1 (0.40–0.99 mm), Group 2 (1.00–2.19 mm), Group 3 (2.20–2.79 mm), and Group 4 (2.80–6.50 mm).
