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In this study, we performed a comprehensive detection of potential pathogens by de novo sequencing using a next-generation DNA sequencer on total RNAs extracted from an autopsy lung of a patient who died of viral pneumonia with A/H1N1/2009.
A comprehensive detection of these alterations is not an inaccessible summit.
A comprehensive detection of immune-checkpoint molecules has been technically challenging due to the lack of robust high-throughput assays that enable a qualitative and quantitative analysis of heterologous interactions between tumor cells and T cells.
To ensure a comprehensive detection of exon-exon junctions, a database of annotated exon-exon junctions (Ensembl version 66) was provided to STAR, a minimum distance was not enforced for the detection of neighboring junctions, and the same criteria (e.g., minimum read coverage) was used to identify both canonical and noncanonical spliced junctions.
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We show here that the integration of the information captured by these different platforms results in a more comprehensive detection of differentially regulated genes and an enhancement of the apparent biological relevance of the findings.
In our opinion, such global approach allows for a more comprehensive detection of damage compared to the analysis of selected damage types or selected makers.
Previous analyses of whole human genomes have shown that SVs affect more base pairs than single point mutations, yet the field has struggled to find a suitable approach for comprehensive detection of such events [ 30].
15 We subsequently developed an additional technology for comprehensive detection of LRs in both BRCA1 and BRCA2.
Since the rice genome was recently sequenced with high accuracy using a japonica cultivar, Nipponbare [ 9], comprehensive detection of SNPs using the Nipponbare sequence as a reference has become an effective tool.
The chromosomal status of each embryo was investigated using microarray comparative genomic hybridisation (array CGH) as described by Fragouli and colleagues (Fragouli et al, 2011), a highly validated method that provides comprehensive detection of aneuploidy in minute samples, including single cells.
The use of SI-labeled metabolites and an HSQC-based chemical shift database would allow the comprehensive detection of large numbers of metabolites in a metabolic network study.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com