Comparing Figure 1a,b it was found that there were no differences between the same compositional samples, except for the relative intensity.
After irradiation, the glass density increased and the SAXS intensity decreased, indicating a compositional homogenization process in the samples subject to a higher dose of irradiation.
Prior to compositional analysis samples were dried to less than 10% moisture and milled to a 2-mm particle size using a bench top or Wiley mill.
Sows were weighed on days 2 and 22. Feed intake of sows was monitored daily, a milk sample was obtained on day 21 for compositional analyses and jugular blood samples were collected on days 2 and 21 to measure prolactin, IGF-I and urea.
*OD measurement was not possible due to flocculation; bold: fermentations that are selected for sample compositional analysis.
Close sample clustering indicates their compositional similarity, whereas distant sample clustering suggests their diverse metabolomic compositions [17].
Similar compositional contrasts between samples FZ01 to FZ08 and samples FZ09 to FZ12 are also apparent in other major and trace element concentrations (K2O, Cr, Ni, Rb, Cs, Ba, Pb, and Th) and in Sr isotope ratios (Table 1).
A significant limitation of NIR analysis is that without samples of known composition to serve as calibration standards, it is impossible to identify specific compositional differences between samples.
Data for these analyses were obtained from compositional and topographic sampling along Sapelo Island, Georgia and South Core Banks, North Carolina.
Our initial challenge, applying a general rule-of thumbs that has been widely used in the industry (based on compositional gradients and sampling horizons dependency), failed to provide understanding into the probable causes of the AOP result variations.
