The discrepancy in findings between the two studies may be explained by the dependency of the oligo dt) capture method on mass spectrometry analysis to identify all enriched proteins from a complex sample, which increases susceptibility for false negatives.
We used a Complex Sample Analysis method to account for the multi stage sampling method and different sample weights [ 26, 27].
32 For a complex sample data with categorical variables we used a four step procedure for multiple imputation.
Glycoproteomics, a sub-proteomic approach that specifically investigates the levels of glycoproteins in a complex sample, has been widely applied for cancer biomarker discovery [ 20- 22].
To account for cluster sampling and sample weight of the DHS [ 24], a complex sample analysis method was deemed appropriate for a more precise estimation of proportion and association [ 25].
A complex sample RNA mixture will offer ample substrate for nonspecific hybridization.
Weighted data were obtained by using a complex sample plan and were submitted to the complex samples analysis in SPSS version 17 for Windows (SPSS Inc., Chicago, IL).
A complex sampling design was adopted for this study and consisted of a combination of probabilistic sampling methods for selecting a representative sample of the population [ 12].
A particular challenge in flow cytometry is to identify rare subpopulations in large, complex samples, for example T cells secreting cytokines in response to antigen stimulation 3, 22, 23; stem cells in peripheral blood 24; or residual cancer cells after therapy 25.
One box filter is used to remove the noise outside the spectra band and reduce the data rate of the complex sampling for the next real-time computations.
