Moreover, in silico assessment of specificity is not sufficient and only an experimental validation can ensure a complete specificity.
This methodology, which requires the complete specificity of all cosmic-ray detector design parameters, is shown to provide approximately unbiased, minimum variance, and normally distributed spectra information for events detected by an instrument having a wide range of commonly used detector response functions.
After the PCR was complete, specificity of each primer pair was confirmed using melt curve analysis.
Dilution of OTUD2 to picomolar concentrations recovered complete specificity of the DUB against the K11 peptide, and even the similar K6 peptide was not hydrolyzed significantly at low enzyme concentrations.
Primer-BLAST (20) analysis to confirm complete specificity of amplicon generation primer pairs was performed using standard stringent parameters: human genome assembly hg19, blast E value 30 000and unintended targets with <6 mismatches were considered.
In all cases, complete specificity of effect on SCM was found, LNEs affecting only lymphoid cell populations, GCEs affecting only the granulocytic cell populations and RCEs affecting only erythroid cells.
After the PCR was complete, specificity of each primer pair was confirmed using melt curve analysis, and all samples run on a 2% ethidium bromide agarose gel with a 50-bp DNA ladder (Invitrogen, Carlsbad, CA) to verify correct product size.
To the best of our knowledge, this essentially complete specificity change of BtsI is the first successful example of such in REases other than Type IIG.
This collection contains a partial or complete specificities for a total of 325 fruit fly TFs.
However, the use of specific primers showed complete specificity (Yersinia and Chlamydia rRNAs were only detected in patients in whom other evidence pointed to the involvement of these organisms) but low sensitivity in the case of Yersinia, because only one out of three patients with ReA thought to be related to Yersinia infection had evidence of the specific rRNA in SF.
