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MSCs were isolated from femurs and tibias of mice, the cells were then plated onto culture plates in a complete medium consisting of Dulbecco's Modified Eagle's Medium (DMEM) (Invitrogen) and 10% fetal bovine serum (Invitrogen) and incubated in a 37°C, 5% CO2 incubator.
A complete medium exchange in both compartments was performed after 24 h of culture.
An in vitro GTT was initiated by instantaneously raising the glucose levels to 11 mM by a complete medium exchange.
Medium samples were collected during a complete medium exchange every 48 h for glucose, insulin and albumin analyses.
Co-substrate utilization of various wastes with complementary characteristics can provide a complete medium for higher hydrogen production.
This all leads to a maximum volume reduction of 10%, as a complete medium exchange was carried out directly after taking the sample at 48 h.
Similar(5)
This procedure revealed a ∼10-fold JAM-A concentration as determined by filter centrifugation of dilutions of recombinant JAM-A in complete medium.
Six hours later, lipofectamine-containing medium was replaced by a fresh complete medium.
This suggests that hydrolysate and seawater formed a nutrient complete medium for PHB production.
Single yeast colonies grown on a synthetic complete medium lacking uracil were cultured overnight in the same fresh liquid medium.
Cells were exposed to 0.5 mg/mL of MTT for 3 h at 37 °C in an appropriate complete medium.
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