The native complex of T25 has a hydrophobic interface with a complementary shape, and that of 1ewy has a modestly electrostatic and shape complementary interface.
To display an overall measure of the interaction frequencies of each amino acid with all the residues of the complementary interface, we calculated the preferences of amino acids in three kinds of interfaces, including TCR-pMHC, Ag-Ab, and protein-protein interfaces.
If we had proposed that this segment of aff4 functions to bind CDK9, we suspect the reviewers would have noted our naïveté about an interaction of a 20-residue helix between molecules in one of 3 crystal complexes that makes a non-complementary interface and contributes less than one kcal per mole to the affinity.
Such a precise pattern of alternating complementary interfaces was not observed in other four-helical bundles, and is likely to be specifically required for the precise architecture of the Kinesin-5 bipolar minifilament.
Computational tools to predict protein protein docking that would allow a rational approach to design novel complementary interfaces are being developed and, although still error-prone [ 13], they may increasingly guide and accelerate the design of functional interfaces between signalling modules.
Despite a wealth of available structural data of complementary interfaces between proteins and domains, our knowledge remains largely descriptive and only a few generalizable patterns of protein and domain interfaces exist [ 12].
Both interactions exhibit properties characteristic of antibody-antigen interactions: high affinity, exquisite specificity contributed by a small number of functional hotspot residues, and the size and properties of the complementary interfaces.
In this approach, charged copolymers are selectively partitioned to the microdroplet interface by a complementary charged surfactant for subsequent supramolecular cross-linking via cucurbit[8]uril.
Herein, we present the assembly of supramolecular microcapsules from aqueous microdroplets driven by electrostatic interactions, whereby charged copolymers are selectively accumulated at the microdroplet interface by a complementary charged surfactant for subsequent supramolecular assembly via CB[8].
