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Based on this exploitation, the existing backward liveness analyses are improved, and a complementary forward liveness analysis is proposed.
To create fluorescently-labeled intrabodies, humanized enhanced green fluorescent protein (EGFP) was PCR-amplified (without a start codon) using a complementary forward primer that introduced an N-terminal (Gly4Ser)4 flexible peptide repeat.
For introducing mutations, CEACAM1 was amplified by polymerase chain reaction from the pIG-NA1B construct using either the common forward primer and a reverse primer containing the desired mutation, or a complementary forward primer containing the mutation and a common reverse primer.
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Rod Thorn, the Nets' president, was also reluctant to take Cousins because he wasn't convinced Cousins, who played center in college, could be a complementary power forward on the frontline with center Brook Lopez.
EAST RUTHERFORD, N.J. - The Nets continued their search for a complementary big forward to center Brook Lopez on Monday by working out the college freshmen DeMarcus Cousins of Kentucky and Derrick Favors of Georgia Tech.
Equimolar quantities of complementary forward and reverse strands were combined and placed in a 95°C water bath for 10 min then slowly cooled to room temperature.
He would be the perfect complementary forward for Derrick Favors, who in two to three years could be dominating Stoudemire around the rim when the Nets of Brooklyn invade Manhattan.
We review the recent progress in systems modelling and omics analysis of the heterocellular heart environment through complementary forward and inverse approaches, illustrating these conceptual and experimental frameworks with case studies from our own research program.
All murine JAK2 (and JAK1) mutants were obtained by PCR reactions with mutagenic complementary forward and reverse primers by the QuickChange Site-Directed Mutagenesis method (Stratagene).
Briefly, complementary forward and reverse oligonucleotides were denatured 2 min at 95°C and placed at room temperature for 30 min to generate double stranded oligonucleotides.
The pEGFP-5BR construct contains the KLF6 putative NLS sequence (PDGRRRVHR) that was cloned EcoRI/BamHI in pEGFP-C3 from annealing of complementary forward and reverse primers (Table S1).
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