Sentence examples for a common reference pool from inspiring English sources
Each amplified sample was hybridized twice against a common reference pool in a dye-swap experiment.
Intensities shown are the mean of all probes for each transcript (113 for ACC1 and 120 for ACC2), after normalization using a common reference pool.
For comparisons among TS and ES (R1) cells, siTcl1-transfected ES cells, ZHTc6 and ZHBTc4 cells, each sample was hybridized against a common reference pool of RNA.
The control samples were "a common reference pool of mRNA".
For these miRNAs, log2 fold changes against a common reference pool were calculated and the distribution was analyzed (Fig. 3a).
Our approach extended the novelty of using a common reference pool to expand the eight channels available in a single iTRAQ experiment by allowing integration of multiple experiments.
Therefore, 800 ng total RNA were hybridized against a common reference (pooled RNA from all samples).
The 24 samples and a common reference pooled sample (made by taking an equal amount of protein from the EV 12 and EV 24 samples) were placed on eight 4-plex iTRAQ runs as described in Additional file 1.
Three hundred nanograms of each Cy3-labelled test sample was competitively hybridised with 300 ng Cy5-labelled common reference pool on a 15 K feature custom microarray, following the manufacturer's instructions (Agilent Technologies, UK).
A total of 20 arrays (4 replicates x 5 tissues) were assayed in the final common reference pool design, with the pool comprising a contribution from each biological sample.
Briefly, each tumour in the independent data set was hybridized twice (dye-swap) against the same common reference pool from the pilot study on an array containing 21.372 probes enriched for genes related to ovarian cancer.
