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Here, we describe a novel protocol for generating highly enriched, functional astrocytes derived from human embryonic stem cells (HESCs), using a combination of developmental cues.
· A combination of developmental stressors, whether nutritional or toxic, could cause effects jointly with similar or other exposures at different times to trigger or exacerbate adverse effects.
These adverse effects, however, "may not be apparent during a latent period which may last from months to years or decades," and they can result from "a combination of developmental stressors…[that] cause effects jointly with similar or other exposures".
Aging in all creatures is inexorable and has been suggested to be a combination of developmental changes, genetic defects, environmental influences, and an inborn genetic aging process (Harman, 1998; Guarente & Kenyon, 2000; Clancy et al., 2001; Tatar et al., 2001; Troen, 2003).
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Signaling specificity is likely to require a combination of several developmental and molecular mechanisms.
The causes of SIDS and SUDI are multi-factorial, due to a combination of environmental, developmental and genetic factors [ 12- 15].
For example, ethanol exposure was limited to embryogenesis, larval or pupal stages, or, alternatively, to a combination of these developmental stages (Table 1).
The data presented here suggest that Drosophila p53 exhibits a combination of both developmental stage-specific and sex-specific antagonistic pleiotropy with regard to life span.
It is also possible the dramatic habituation defects in rpm-1 mutants could stem from a combination of both developmental abnormalities.
A combination of initial developmental differences and lifelong allometric growth of structures could potentially also explain the variation in the bivariate allometric relationships between brain structures in Tanganyikan cichlids and the next step is to analyze potential ecological, behavioral and sexually selected correlates of brain structures.
Indeed, Emperor Trump has no clothes, and he is little more than a toxic combination of developmental deficiencies – the vocabulary of a third grader, the temperament of a toddler, and the mental capacity of a fetus bottled in formaldehyde that Carly Fiorina pinned on Planned Parenthood.
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