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There is also a cold solution.
Arenediazonium chloride (5 mmol), which was prepared from aromatic amines (5 mmol), hydrochloric acid (6 N, 6 ml), and sodium nitrite (0.35 g, 5 mmol), then it was added dropwise with stirring to a cold solution of a mixture of (25) (1.35 g, 5 mmol) and sodium acetate trihydrate (1.3 g 10 mmol) in ethanol (50 ml).
The penis was then soaked in a cold solution of 10% KOH, and slide-mounted using a small amount of KY-Jelly immersed in filtered 70% EtOH.
Frozen tissue sections of the tumors were fixed in a cold solution of methanol:acetic acid (3:1) for one hour.
Fully expanded Arabidopsis leaves were collected at end of day, cut into 2 x 2 mm pieces, and immediately fixed with a cold solution of glutaraldehyde.
Connect the catheter to a syringe and inject 15-20 ml of a cold solution of colagenase P (1 mg/ml) in HBSS until the pancreas is full.
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The endovascular cooling catheter contains a circulating cold solution, which is maintained at a controlled temperature; this method can easily achieve a cooling rate of 1.5 4.5 °C/h [52].
The endovascular cooling catheter contains a circulating cold solution, which is maintained at a controlled temperature; this method can easily achieve a cooling rate of 1.5 4.5 °C/h [ 52].
To a stirred cold solution (0°C) of 500 mg of sodium hyaluronate in 10 ml of toluene were added a catalytic amount of 4-dimethylaminopyridine (DMAP) and an excess of acetic anhydride.
An aqueous stock solution of p-nitrophenol or p-nitroaniline (0.3 ml of 2 mM) was mixed with distilled water (1.4 ml) and then an ice cold solution of sodium borohydride (1 ml of 0.03 M) was added.
Carbon disulphide (20 mmol) was added drop wise to an ice cold solution of potassium hydroxide (10 mmol) in absolute alcohol (30 ml) containing acetohydrazide 14 (1.25 g, 5 mmol).
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