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However, careful addition of GP, a weak base to a cold acidic chitosan solution, neutralizes the positively charged ammonium groups on chitosan chains gradually.
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The bottom is a breathless place — cold, acidic, anaerobic — with no oxygen to decompose the willow branches or the small, still faces of the foxes interred there.
The mixture was then poured dropwise into a large excess of cold acidic water (5%%).
Mike may have found the cold, acidic water seeping through the fresh rips in his neoprene waders equally brisk and refreshing, but being the quiet type, I supposed he was savoring the joy of the moment privately.
As control, cells were infected in presence of equal volumes of DMSO setting 100% of infection; instead, to confirm viral attachment but not viral entry at 4°C, after incubation cells were treated for two minutes with cold acidic glycine (100 mM glycine, 150 mM NaCl, pH 3) to inactivate attached virus, resulting in 100% inhibition of infection.
For detection of the pancreatic insulin content, whole pancreases were homogenised in ice-cold acidic ethanol (0.1 mol/l HCl in 70% ethanol) and incubated for 24 h at 4°C.
We find the most lovely, acidic asides: "On a cold day, in a drafty room, chilled cucumber soup is about as welcome as a swarm of wasps at a bat mitzvah".
Prior to extrusion of a lava dome, an acidic cold lake filled the bottom of the crater.
Evaporative stimuli such as a triple syringe air jet or cold windy weather results in desiccation of the dentin surface and is thought to increase in the outward flow of fluid in the tubules, as does a cold thermal stimulus and osmotic stimuli such as sugar or acidic fluid [ 36].
His performance is matched in acidic power by Katrin Cartlidge's portrayal of Jane Livingstone, a cold, snippy journalist whose anything-to-get-the-story attitude embodies the unquestioned sense of entitlement that so many television journalists bring to their work.
Her team found organic items like caches of corn and beans in a cold cellar, parts of floorboards and pieces of cloth, animal bone and antlers, which typically deteriorate quickly in Connecticut's acidic soil.
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