Sentence examples for a cloning vector from inspiring English sources

The fragment of DNA to be amplified is first inserted into a cloning vector.

AiiA gene was cloned to a cloning vector pUC and sequenced.

A genomic library of the hyperthermophilic archaeon Sulfolobus solfataricus strain MT4 was constructed in Escherichia coli using a cloning vector not designed for heterologous gene expression.

A cloning vector (length not to scale) that shares 30-bp terminal sequence overlaps (black cross) with the target DNA at both ends is ligated to the target segment in a Gibson assembly mix.

A cloning vector for lactic acid bacteria was developed on the basis of the pC7 replicon, into which were inserted an erythromycin resistance gene as a marker, multiple cloning sites, and Escherichia coli ColE1 replication origin.

Together, our findings strongly suggest that the strategy herein developed combining heterologous expression using a cloning vector carrying the pAMβ1 replication origin and experimental designs optimization can be generalized for recombinant proteins production in Bacillus.

Because pDG780 lacked available restriction enzyme sites to clone some of these fragments, the vectors pUC19, pEG-28a or pMD18-T Simple vector (a T-A cloning vector with no multiple cloning sites; TaKaRa Biotechnology [Dalian] Co., Ltd) were used to obtain sites suitable for cloning in pDG780.

Amplified PCR products were ligated to a T/A cloning vector (pTZ57R/T, Fermentas, WI, USA) to generate pTZ-U6 lhRNA and shRNA plasmids.

These fragments were inserted into a T/A cloning vector, and the resultant SSH library was used for sequence analysis.

These amplicons were subcloned into a T/A cloning vector and ∼50 clones per region were screened via restriction-enzyme digest and DNA sequencing for variations in exon structure.

Prior to ligation into pCR®2.1 the subtracted PCR cDNA products were subjected to an additional incubation of 1 hour at 72°C with dATP and Taq polymerase (TaKaRa; Gennevilliers, France) to ensure that the majority of the PCR fragments contain "A-overhangs" for an efficient cloning into the T/A cloning vector.