The phrase "a cloning plasmid" is correct and usable in written English.
It can be used in scientific contexts, particularly in molecular biology, to refer to a plasmid designed for cloning purposes.
Example: "Researchers utilized a cloning plasmid to insert the desired gene into the bacterial host."
Alternatives: "a plasmid for cloning" or "a plasmid designed for cloning".
For quantification, a standard curve (log-linear R2 > 0.97) was generated using a purified, linearized, and quantified archaeal amo A clone plasmid in a dilution series that spanned from 101 to 109 gene copies per reaction.
A sequence error was defined as discordance in an equivalent position between the reference and control (from the 49 healthy individuals and a cloned plasmid vector).
Sensitivity was determined using a cloned plasmid containing the gltA gene from the Ehrlichia sp. Reaction conditions were optimized to ensure a sensitivity of at least 10 gene copies.
The PCR products of SAG5D gene was cloned into a pEASY-T1 vector (TransGen Biotech, China) to generate a recombinant cloning plasmid.
The pellet was resuspended in 500 μL deionized H2O containing 100 μg/mL RNAse A. Standard cloning, plasmid isolation and bacterial transformations were performed as previously described (Sambrook and Russel 2001).
A clone, harboring plasmid pBK-CMV amy13A, showing a blue halo was isolated and the plasmid extracted, containing an insert of about 5 kb.
The PCR products were quantitated by hybridization with a Taqman probe (genomic coordinates 4218 4189) and converted to genome copies by comparison with a standard curve of cloned plasmid template amplified in parallel.
Escherichia coli DH10B (Life Technologies) was used as a bacterial cloning and plasmid propagation host.
We cloned a plasmid containing a sgRNA scaffold under the control of a U6 or tRNA promoter into the pGEM-T Easy vector (Supplementary Fig. S1B).
