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We have investigated the influence of important experimental and environmental factors on the cloning outcome in a considerably large data set comprising over 270 porcine nuclear transfer experiments.
We have employed robust linear models, requiring minimal distribution assumptions adjusted to the underlying empirical distribution of the cloning outcome, as a straightforward approach to determine the statistically significant part of the network of factors affecting pig cloning.
The effect of selection of SCNT embryos on the cloning outcome is shown in Table 7.
We assessed the influence on the cloning outcome of five factors: the season the embryo transfer (ET) was performed in, the type of genetic modification, the donor cell source, the number of cloning rounds, and selection of SCNT embryos for development before transfer to the recipient.
The outcome of inserting a clone is an empirical matter, however, and rests on the rate at which the parasite environment causes loss of adaptation in the host.
Knowledge of the complete pig genome sequence will provide a more detailed understanding of this epigenetic regulation at specific regulatory regions of genes and enable swine-specific refinement of SCNT treatments to improve cloning outcomes [ 21].
A clone.
A clone does not.
Wiener is a clone!
The clone was somehow no longer quite a clone.
"It could be a clone.
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