The aim of this study was to design a microarray targeting methanogens found in anaerobic digesters, and to apply this chip together with a cloning approach to investigate the methanogenic community present in an anaerobic digester.
A cloning approach using restriction endonucleases LguI and/or Eco81I enabled a step-by-step continuous ligation of two fragments in vector pQE-30-LE.
Up to now, miRNA identification in fruits using a cloning approach is rare except in tomato [ 23] and grape [ 60].
The first studies of p53 alterations in the urine used a cloning approach followed by sequencing to confirm the presence of mutations (Sidransky et al, 1991).
We designed a cloning approach based on synthetic overlapping primers and a PCR assembly strategy to construct a synthetic hIL-25 cDNA fragment without the signal peptide sequence (Xiong et al. 2004).
We sequenced the Florida strain to completion using a BAC-based clone by clone approach, and obtained high coverage genome sequence data for three additional strains.
In a continuation of the germline clone approach for meiotic mutant isolation, we sought to identify novel fertile meiotic mutants on the X chromosome with strong effects on either chromosome segregation or recombination.
Although the high number of mutations per genome might be considered as a disadvantage since more back-crosses will be necessary to reduce total mutation number and identify genes potentially associated with a particular phenotype via a positional cloning approach, it also presents a number of advantages.
The calcium-transporting ATPase 2C2 (and2cMAFand cMAF inducing protein (CMIP) genes, both on chromosome 16q, were identified as SLI candidates by a positional cloning approach, which involved a genome-wide linkage study followed by a targeted high-density association investigation [ 53, 67- 70].
SAIL does the same with the CloNe approach.
A high coverage p53 shRNA library was prepared using a novel cloning approach where random sequences derived from the p53 mRNA sequence are converted into a form that encodes a hairpin structure and cloned into a U6 promoter cassette within a self-inactivating retroviral vector (Additional file 5: Figure S5).
